Isolation and characterization of a Solanum tuberosum subtilisin-like protein with caspase-3 activity (StSBTc-3).
Identifieur interne : 000E31 ( Main/Exploration ); précédent : 000E30; suivant : 000E32Isolation and characterization of a Solanum tuberosum subtilisin-like protein with caspase-3 activity (StSBTc-3).
Auteurs : María Belén Fernández [Argentine] ; Gustavo Raúl Daleo [Argentine] ; María Gabriela Guevara [Argentine]Source :
- Plant physiology and biochemistry : PPB [ 1873-2690 ] ; 2015.
Descripteurs français
- KwdFr :
- Apoptose (génétique), Caspase-3 (génétique), Caspase-3 (métabolisme), Cellules cultivées (MeSH), Cellules végétales (métabolisme), Données de séquences moléculaires (MeSH), Feuilles de plante (génétique), Feuilles de plante (microbiologie), Feuilles de plante (métabolisme), Interactions hôte-pathogène (MeSH), Lycopersicon esculentum (cytologie), Masse moléculaire (MeSH), Microscopie de fluorescence (MeSH), Phylogenèse (MeSH), Phytophthora infestans (physiologie), Protéines végétales (composition chimique), Protéines végétales (génétique), Protéines végétales (métabolisme), Similitude de séquences d'acides aminés (MeSH), Solanum tuberosum (génétique), Solanum tuberosum (microbiologie), Solanum tuberosum (métabolisme), Spectrométrie de masse MALDI (MeSH), Subtilisine (classification), Subtilisine (génétique), Subtilisine (métabolisme), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Électrophorèse sur gel de polyacrylamide (MeSH).
- MESH :
- classification : Subtilisine.
- composition chimique : Protéines végétales.
- cytologie : Lycopersicon esculentum.
- génétique : Apoptose, Caspase-3, Feuilles de plante, Protéines végétales, Solanum tuberosum, Subtilisine.
- microbiologie : Feuilles de plante, Solanum tuberosum.
- métabolisme : Caspase-3, Cellules végétales, Feuilles de plante, Protéines végétales, Solanum tuberosum, Subtilisine.
- physiologie : Phytophthora infestans.
- Cellules cultivées, Données de séquences moléculaires, Interactions hôte-pathogène, Masse moléculaire, Microscopie de fluorescence, Phylogenèse, Similitude de séquences d'acides aminés, Spectrométrie de masse MALDI, Séquence d'acides aminés, Séquence nucléotidique, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Apoptosis (genetics), Base Sequence (MeSH), Caspase 3 (genetics), Caspase 3 (metabolism), Cells, Cultured (MeSH), Electrophoresis, Polyacrylamide Gel (MeSH), Host-Pathogen Interactions (MeSH), Lycopersicon esculentum (cytology), Microscopy, Fluorescence (MeSH), Molecular Sequence Data (MeSH), Molecular Weight (MeSH), Phylogeny (MeSH), Phytophthora infestans (physiology), Plant Cells (metabolism), Plant Leaves (genetics), Plant Leaves (metabolism), Plant Leaves (microbiology), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Sequence Homology, Amino Acid (MeSH), Solanum tuberosum (genetics), Solanum tuberosum (metabolism), Solanum tuberosum (microbiology), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH), Subtilisin (classification), Subtilisin (genetics), Subtilisin (metabolism).
- MESH :
- chemical , chemistry : Plant Proteins.
- chemical , classification : Subtilisin.
- chemical , genetics : Caspase 3, Plant Proteins, Subtilisin.
- cytology : Lycopersicon esculentum.
- genetics : Apoptosis, Plant Leaves, Solanum tuberosum.
- chemical , metabolism : Caspase 3, Plant Cells, Plant Leaves, Plant Proteins, Solanum tuberosum, Subtilisin.
- microbiology : Plant Leaves, Solanum tuberosum.
- physiology : Phytophthora infestans.
- Amino Acid Sequence, Base Sequence, Cells, Cultured, Electrophoresis, Polyacrylamide Gel, Host-Pathogen Interactions, Microscopy, Fluorescence, Molecular Sequence Data, Molecular Weight, Phylogeny, Sequence Homology, Amino Acid, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
Abstract
Plant proteases with caspase-like enzymatic activity have been widely studied during the last decade. Previously, we have reported the presence and induction of caspase-3 like activity in the apoplast of potato leaves during Solanum tuberosum- Phytophthora infestans interaction. In this work we have purified and identified a potato extracellular protease with caspase-3 like enzymatic activity from potato leaves infected with P. infestans. Results obtained from the size exclusion chromatography show that the isolated protease is a monomeric enzyme with an estimated molecular weight of 70 kDa approximately. Purified protease was analyzed by MALDI-TOF MS, showing a 100% of sequence identity with the deduced amino acid sequence of a putative subtilisin-like protease from S. tuberosum (Solgenomics protein ID: PGSC0003DMP400018521). For this reason the isolated protease was named as StSBTc-3. This report constitutes the first evidence of isolation and identification of a plant subtilisin-like protease with caspase-3 like enzymatic activity. In order to elucidate the possible function of StSBTc-3 during plant pathogen interaction, we demonstrate that like animal caspase-3, StSBTc-3 is able to produce in vitro cytoplasm shrinkage in plant cells and to induce plant cell death. This result suggest that, StSBTc-3 could exert a caspase executer function during potato- P. infestans interaction, resulting in the restriction of the pathogen spread during plant-pathogen interaction.
DOI: 10.1016/j.plaphy.2014.12.001
PubMed: 25486023
Affiliations:
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Electronic address: mbfernan@mdp.edu.ar.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata-CONICET, CC 1245, 7600 Mar del Plata</wicri:regionArea><wicri:noRegion>7600 Mar del Plata</wicri:noRegion></affiliation></author><author><name sortKey="Daleo, Gustavo Raul" sort="Daleo, Gustavo Raul" uniqKey="Daleo G" first="Gustavo Raúl" last="Daleo">Gustavo Raúl Daleo</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata-CONICET, CC 1245, 7600 Mar del Plata, Argentina. Electronic address: grdaleo@mdp.edu.ar.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata-CONICET, CC 1245, 7600 Mar del Plata</wicri:regionArea><wicri:noRegion>7600 Mar del Plata</wicri:noRegion></affiliation></author><author><name sortKey="Guevara, Maria Gabriela" sort="Guevara, Maria Gabriela" uniqKey="Guevara M" first="María Gabriela" last="Guevara">María Gabriela Guevara</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata-CONICET, CC 1245, 7600 Mar del Plata, Argentina. Electronic address: gguevara@mdp.edu.ar.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata-CONICET, CC 1245, 7600 Mar del Plata</wicri:regionArea><wicri:noRegion>7600 Mar del Plata</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant physiology and biochemistry : PPB</title><idno type="eISSN">1873-2690</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Apoptosis (genetics)</term><term>Base Sequence (MeSH)</term><term>Caspase 3 (genetics)</term><term>Caspase 3 (metabolism)</term><term>Cells, Cultured (MeSH)</term><term>Electrophoresis, Polyacrylamide Gel (MeSH)</term><term>Host-Pathogen Interactions (MeSH)</term><term>Lycopersicon esculentum (cytology)</term><term>Microscopy, Fluorescence (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Molecular Weight (MeSH)</term><term>Phylogeny (MeSH)</term><term>Phytophthora infestans (physiology)</term><term>Plant Cells (metabolism)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (metabolism)</term><term>Plant Leaves (microbiology)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Sequence Homology, Amino Acid (MeSH)</term><term>Solanum tuberosum (genetics)</term><term>Solanum tuberosum (metabolism)</term><term>Solanum tuberosum (microbiology)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH)</term><term>Subtilisin (classification)</term><term>Subtilisin (genetics)</term><term>Subtilisin (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Apoptose (génétique)</term><term>Caspase-3 (génétique)</term><term>Caspase-3 (métabolisme)</term><term>Cellules cultivées (MeSH)</term><term>Cellules végétales (métabolisme)</term><term>Données de séquences moléculaires (MeSH)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (microbiologie)</term><term>Feuilles de plante (métabolisme)</term><term>Interactions hôte-pathogène (MeSH)</term><term>Lycopersicon esculentum (cytologie)</term><term>Masse moléculaire (MeSH)</term><term>Microscopie de fluorescence (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Phytophthora infestans (physiologie)</term><term>Protéines végétales (composition chimique)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Solanum tuberosum (génétique)</term><term>Solanum tuberosum (microbiologie)</term><term>Solanum tuberosum (métabolisme)</term><term>Spectrométrie de masse MALDI (MeSH)</term><term>Subtilisine (classification)</term><term>Subtilisine (génétique)</term><term>Subtilisine (métabolisme)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Électrophorèse sur gel de polyacrylamide (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Subtilisin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Caspase 3</term><term>Plant Proteins</term><term>Subtilisin</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="fr"><term>Subtilisine</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Lycopersicon esculentum</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Lycopersicon esculentum</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Apoptosis</term><term>Plant Leaves</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Apoptose</term><term>Caspase-3</term><term>Feuilles de plante</term><term>Protéines végétales</term><term>Solanum tuberosum</term><term>Subtilisine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Caspase 3</term><term>Plant Cells</term><term>Plant Leaves</term><term>Plant Proteins</term><term>Solanum tuberosum</term><term>Subtilisin</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Leaves</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Caspase-3</term><term>Cellules végétales</term><term>Feuilles de plante</term><term>Protéines végétales</term><term>Solanum tuberosum</term><term>Subtilisine</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cells, Cultured</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Host-Pathogen Interactions</term><term>Microscopy, Fluorescence</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Phylogeny</term><term>Sequence Homology, Amino Acid</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term><term>Données de séquences moléculaires</term><term>Interactions hôte-pathogène</term><term>Masse moléculaire</term><term>Microscopie de fluorescence</term><term>Phylogenèse</term><term>Similitude de séquences d'acides aminés</term><term>Spectrométrie de masse MALDI</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plant proteases with caspase-like enzymatic activity have been widely studied during the last decade. Previously, we have reported the presence and induction of caspase-3 like activity in the apoplast of potato leaves during Solanum tuberosum- Phytophthora infestans interaction. In this work we have purified and identified a potato extracellular protease with caspase-3 like enzymatic activity from potato leaves infected with P. infestans. Results obtained from the size exclusion chromatography show that the isolated protease is a monomeric enzyme with an estimated molecular weight of 70 kDa approximately. Purified protease was analyzed by MALDI-TOF MS, showing a 100% of sequence identity with the deduced amino acid sequence of a putative subtilisin-like protease from S. tuberosum (Solgenomics protein ID: PGSC0003DMP400018521). For this reason the isolated protease was named as StSBTc-3. This report constitutes the first evidence of isolation and identification of a plant subtilisin-like protease with caspase-3 like enzymatic activity. In order to elucidate the possible function of StSBTc-3 during plant pathogen interaction, we demonstrate that like animal caspase-3, StSBTc-3 is able to produce in vitro cytoplasm shrinkage in plant cells and to induce plant cell death. This result suggest that, StSBTc-3 could exert a caspase executer function during potato- P. infestans interaction, resulting in the restriction of the pathogen spread during plant-pathogen interaction.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25486023</PMID><DateCompleted><Year>2015</Year><Month>09</Month><Day>17</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2690</ISSN><JournalIssue CitedMedium="Internet"><Volume>86</Volume><PubDate><Year>2015</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Plant physiology and biochemistry : PPB</Title><ISOAbbreviation>Plant Physiol Biochem</ISOAbbreviation></Journal><ArticleTitle>Isolation and characterization of a Solanum tuberosum subtilisin-like protein with caspase-3 activity (StSBTc-3).</ArticleTitle><Pagination><MedlinePgn>137-146</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0981-9428(14)00364-7</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.plaphy.2014.12.001</ELocationID><Abstract><AbstractText>Plant proteases with caspase-like enzymatic activity have been widely studied during the last decade. Previously, we have reported the presence and induction of caspase-3 like activity in the apoplast of potato leaves during Solanum tuberosum- Phytophthora infestans interaction. In this work we have purified and identified a potato extracellular protease with caspase-3 like enzymatic activity from potato leaves infected with P. infestans. Results obtained from the size exclusion chromatography show that the isolated protease is a monomeric enzyme with an estimated molecular weight of 70 kDa approximately. Purified protease was analyzed by MALDI-TOF MS, showing a 100% of sequence identity with the deduced amino acid sequence of a putative subtilisin-like protease from S. tuberosum (Solgenomics protein ID: PGSC0003DMP400018521). For this reason the isolated protease was named as StSBTc-3. This report constitutes the first evidence of isolation and identification of a plant subtilisin-like protease with caspase-3 like enzymatic activity. In order to elucidate the possible function of StSBTc-3 during plant pathogen interaction, we demonstrate that like animal caspase-3, StSBTc-3 is able to produce in vitro cytoplasm shrinkage in plant cells and to induce plant cell death. This result suggest that, StSBTc-3 could exert a caspase executer function during potato- P. infestans interaction, resulting in the restriction of the pathogen spread during plant-pathogen interaction.</AbstractText><CopyrightInformation>Copyright © 2014 Elsevier Masson SAS. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fernández</LastName><ForeName>María Belén</ForeName><Initials>MB</Initials><AffiliationInfo><Affiliation>Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata-CONICET, CC 1245, 7600 Mar del Plata, Argentina. Electronic address: mbfernan@mdp.edu.ar.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Daleo</LastName><ForeName>Gustavo Raúl</ForeName><Initials>GR</Initials><AffiliationInfo><Affiliation>Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata-CONICET, CC 1245, 7600 Mar del Plata, Argentina. Electronic address: grdaleo@mdp.edu.ar.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guevara</LastName><ForeName>María Gabriela</ForeName><Initials>MG</Initials><AffiliationInfo><Affiliation>Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata-CONICET, CC 1245, 7600 Mar del Plata, Argentina. Electronic address: gguevara@mdp.edu.ar.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>12</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>Plant Physiol Biochem</MedlineTA><NlmUniqueID>9882449</NlmUniqueID><ISSNLinking>0981-9428</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.21.62</RegistryNumber><NameOfSubstance UI="D020860">Subtilisin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.22.-</RegistryNumber><NameOfSubstance UI="D053148">Caspase 3</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017209" MajorTopicYN="N">Apoptosis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053148" MajorTopicYN="N">Caspase 3</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008856" MajorTopicYN="N">Microscopy, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008970" MajorTopicYN="N">Molecular Weight</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059828" MajorTopicYN="N">Plant Cells</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019032" MajorTopicYN="N">Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020860" MajorTopicYN="N">Subtilisin</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Apoplast</Keyword><Keyword MajorTopicYN="N">DEVDase</Keyword><Keyword MajorTopicYN="N">Plant–pathogen interaction</Keyword><Keyword MajorTopicYN="N">Solanum tuberosum</Keyword><Keyword MajorTopicYN="N">Subtilisin like protein</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>08</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>12</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>12</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>12</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>9</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25486023</ArticleId><ArticleId IdType="pii">S0981-9428(14)00364-7</ArticleId><ArticleId IdType="doi">10.1016/j.plaphy.2014.12.001</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Argentine</li></country></list><tree><country name="Argentine"><noRegion><name sortKey="Fernandez, Maria Belen" sort="Fernandez, Maria Belen" uniqKey="Fernandez M" first="María Belén" last="Fernández">María Belén Fernández</name></noRegion><name sortKey="Daleo, Gustavo Raul" sort="Daleo, Gustavo Raul" uniqKey="Daleo G" first="Gustavo Raúl" last="Daleo">Gustavo Raúl Daleo</name><name sortKey="Guevara, Maria Gabriela" sort="Guevara, Maria Gabriela" uniqKey="Guevara M" first="María Gabriela" last="Guevara">María Gabriela Guevara</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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